RNAseq Processing

This module accepts RNAseq files and converts them into a feature count table for downstream statistical and functional analysis.

  • For RNAseq from organisms without reference genome, ExpressAnalyst uses our Seq2Fun algorithm to perform sensitive translated searches to identify and quantify homologous protein sequences using comprehensive databases for ~30 taxonomic groups covering >600 organisms.
  • For RNAseq from species with reference genomes, ExpressAnalyst uses the Kallisto algorithm with built-in reference genomes for ~20 common organisms.

Since uploading large files can be very time consuming, two different options are offered:

Local processing
We highly recommend using the Docker version for local RNAseq processing. The pipeline can run well on a modern laptop or workstation with ≥8G RAM and 100G free disc space
Online processing
  • You need to register an account first.
  • The maximum uploading time per session is 4 hour; the maximum storage per user is 30 GB.
  • You may need to wait for your turn to upload data if there are many users.
Processing ....
Your session is about to expire!

You will be logged off in seconds.

Do you want to continue your session?

Download